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Using the Studio One Audio Inputs and Outputs

All editions of the Studio One hardware includes two 1/4" audio inputs and two 1/4" audio outputs. These can all be found labeled on the back of the unit.

To ensure your Studio One audio setup is configured correctly, go to Control Panel and open the Sound settings. You will want to ensure the following:

  • Under the Playback tab, set Speakers: Realtek High Definition Audio as the Default Device. This will allow for the headphone jack in the front of the unit to work so that you can monitor audio from Studio.
  • Also under the Playback tab, set U24XL with SPDIF I/O as the Default Communication Device to allow the ¼” output jacks of the Studio One unit to playback the Program Output of the Studio program at all times.
  • Under the Recording tab, set Line: U24XL with SPDIF I/O as the default device to bring your ¼" audio inputs into Studio.

The 1/4" Out plugs will output Program audio (i.e. audio coming through Stream Out/Record Out in Studio's audio mixer). Keep in mind these outputs are stereo by design, so you will hear Channel 1 through the L output and Channel 2 through the R output.


Plug in your audio source to the inputs (bottom ports). After you launch Studio, go to the Inputs tab in the lower right corner. Select Add Input > Audio Only. Select the 1/4" Input(s).

Troubleshooting: Not Seeing 1/4" Input in Studio Settings?

First, make sure you are running Studio version 5.2 or later, as this version resolves an issue with detecting the drivers for the card.


If Studio still does not detect your audio inputs after updating, you may need to rollback the audio device's drivers to the Windows default driver.


To do this, navigate to Control Panel > Uninstall a program, and uninstall ESI U 24 XL USB Driver version v2.6.0.0. You will need to restart your system after doing this.

Open Control Panel > Device Manager, open the Sound, video and game controllers option and find U24XL with SPDIF I/O. Right-click it and select Properties.

Select the Drivers tab. If the Driver Provider says "ESI" and the Driver Version says "2.6.0," then select Roll Back Driver. This will remove the ESI driver in favor of the Microsoft driver version 10.0.17134.1.

Restart your system again. When you launch Studio, create a new project to start with a fresh audio configuration.


If the issue persists, you can try going back to the Driver window (shown above) and this time select Disable Device and restart your system. When Windows relaunches, go back to Device Manager, find U24XL with SPDIF I/O, right click it and re-enable the device. You will need to restart again and we recommend creating a new Studio project.


If disabling the device does not resolve the issue still, you may again go back to the Driver window and select Uninstall Device. When you restart your machine, the device will reinstall. Relaunch Studio and create a new project.


If the issue still persists, please contact us.


The source will automatically be labeled Audio 1 and appear in both the Inputs list and the Audio Mixer.

You can adjust the channel input into Studio by navigating to the Inputs tab, selecting the gearwheel that corresponds to Audio 1, and then selecting Audio on the left side.


You will see both audio channels; Input 1 is the Left (L) input, Input 2 is the Right (R) input. Each input can be configured to send audio out to either one channel or both channels.


For example, if your audio source is only plugged into the R input on the Studio One unit, you may want to configure Input 2 to send audio to both the L and R channels so that your viewers will hear audio from both sides rather than only one channel.

Both channels will be reflected inside Studio's Audio Mixer.

If you are using both 1/4" inputs, you can keep each input separate by selecting L under Input 1 and R under Input 2, creating a stereo audio output to your stream.

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